Fig. 5
From: Bardoxolone methyl inhibits the infection of rabies virus via Nrf2 pathway activation in vitro
CDDO-Me inhibited different RABV strains via the Nrf2-associated signaling pathway in N2a cells. Nrf2 pathway-associated protein levels were analyzed by western blotting after the infection of N2a cells with SC16 A, CVS D or CTN G at an MOI of 10 and treatment with CDDO-Me (0.5 μM) for 24 h. Virus-infected cells that were treated with DMSO served as a control. Data are representative of at least three independent experiments. B, E, H The results of quantitative analyses of the relative signal densities of Nrf2, phospho-SQSTM1, SQSTM1, Keap1, HO-1, and NQO1 after normalization to the β-actin signal density at the indicated times are shown on the right. C, F, I Culture supernatants were sampled, and the viral titers of the culture supernatants were measured. The data shown represent the mean and standard deviation from two independent experiments that were performed in triplicate. The mean and standard deviation are shown. Statistical analysis was carried out using one-way ANOVA with Dunnett's post hoc test (*p < 0.05, **p < 0.01, ***p < 0.001)