Fig. 4
From: Bardoxolone methyl inhibits the infection of rabies virus via Nrf2 pathway activation in vitro
Activation of the Nrf2-associated signaling pathway by CDDO-Me at different concentrations upon infection with different RABV strains in N2a cells. N2a cells were infected with SC16 A, CVS-11 C or CTN E at an MOI of 10 and treated with different doses of CDDO-Me (0.05 μM, 0.1 μM, 0.2 μM or 0.5 μM) diluted in culture medium for 24 h in triplicate. Virus-infected cells that were treated with DMSO served as a control. Then, the cells were lysed, and the expression levels of Nrf2, phospho-SQSTM1, SQSTM1, Keap1, HO-1, and NQO1 were examined by western blotting. Data are representative of at least three independent experiments. B, D, F) The results of quantitative analysis of the relative signal densities of Nrf2, phospho-SQSTM1, SQSTM1, Keap1, HO-1, and NQO1 after normalization to the β-actin signal density at the indicated times are shown on the right. Each test was performed in triplicate. Graphical data denote the mean ± SD. Statistical significance was assessed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001