Fig. 3
From: PREB inhibits the replication of prototype foamy virus by affecting its transcription
Inhibition of Tas-mediated transactivation of PFV LTR and IP by PREB. (A) Integrated proviral DNA was measured as described in Materials and Methods. (B and C) HEK293T cells were transfected with PFV LTR-Luc (B) or IP-Luc (C) and pCMV-β-gal, combined with Tas and increasing amount of PREB. After 48 h, luciferase activity was measured. The remaining cell lysates were collected for Western blot analysis. (D and E) HT1080 cells were transfected with PFV LTR-Luc (D) or IP-Luc (E) and pCMV-β-gal, combined with Tas and PREB. After 48 h, luciferase activity was measured. The remaining cell lysates were collected for Western blot analysis. * P<0.05, ** P<0.01, **** P<0.0001