Fig. 2

Inhibition of PFV replication by PREB. (A–C) HT1080 cells were infected with PFV (MOI = 0.5) after transfected with PREB or empty vector for 8 h. After 40 h, 600 µl of supernatants (A) or 1/10 infected HT1080 cells (B) were co-cultured with PFVL cells to determine viral titers by luciferase assay. (C) Western blotting was used to detect viral protein expression level. (D–F) PFV (MOI = 0.5) infected HT1080-shControl and HT1080-shPREB cells. After 8 h, HT1080 cells were transfected PREB or empty vector. After 48 h, 600 µl of supernatants (D) or 1/10 infected HT1080 cells (E) were co-cultured with PFVL cells, the luciferase activity was measured 48 h later. (F) The rest HT1080 cells were lysed for Western blot analysis. *P<0.05, **P<0.01