Fig. 6
From: Dysregulation of intracellular redox homeostasis by the SARS-CoV-2 ORF6 protein
ORF6 controls p38 MAPK activity on NRF2 phosphorylation. The cytoplasmic content of (A) Protein Kinase C (PKC) and Glycogen Synthase Kinase 3-beta (GSK-3b) or (B) p38 Mitogen-Activated Protein Kinase (MAPK), total or phosphorylated (pp38), is investigated in HEK-293T cells expressing either wt-ORF6 or the previously described mutant versions. Densitometric analysis of immunoreactive protein bands is performed by ImageJ software by normalization with relative actin loading controls. The results are plotted as the mean fold change in target proteins with respect to the empty plasmid-transfected control from three (n = 3) independent experiments ± standard deviations (SD). Significance is determined with respect to the negative control (Ctr-), transfected with empty plasmid, sample as ***p < 0.0005; **p < 0.005; *p < 0.05