Fig. 4
From: Dysregulation of intracellular redox homeostasis by the SARS-CoV-2 ORF6 protein
ORF6 protein hinders scavenger protein content. The effect of SARS-CoV-2 ORF6 protein, wt- or mutated variants, on the cellular antioxidant response is further analysed by immunoblotting for (A) NRF2 and (B) G6PD and HO-1 on 50 μg of whole cell lysates (WCL) of transfected HEK-293T cells. (C) The effects of ORF6 protein mutants on endogenous NRF2 expression is further investigated on WCL of A549 cells expressing ORF6 protein variants. The loading control is represented by immuno-detection of actin protein. Densitometric analysis of reactive bands is performed by ImageJ software and the results are plotted as the mean fold change in target proteins, normalized with respect to relative actin levels, from (n = 3) independent experiments, performed in duplicate, ± SD. Significance is determined with respect to the negative control (Ctr-), transfected with empty plasmid, sample as **p < 0.005; *p < 0.05