Fig. 4
From: Brequinar inhibits African swine fever virus replication in vitro by activating ferroptosis
Brequinar inhibits ASFV infection in pre-, co-, and post-treatment. Brequinar was mixed with ASFV (MOI = 1) at 37℃ (direct interaction). After 1 h, the virus was separated from brequinar by ultrafiltration centrifugation and then resuspended to infect PAMs for 2 h. PAMs were treated with brequinar for 2 h prior to ASFV infection (pre-treatment). Brequinar was mixed with ASFV and then incubated for 2 h (co-treatment) on PAMs. PAMs were infected with ASFV for 2 h (post-treatment) and then treated with brequinar. After 48 h, the cells were harvested and detected by Western blotting (A) and IFA (C) assays. (B) The p30 and Actin band intensity was quantified using Image J software. Statistical significance is denoted by ***P < 0.001 compared to the DMSO-treated control