Fig. 3
From: Brequinar inhibits African swine fever virus replication in vitro by activating ferroptosis
The antiviral effect of brequinar on ASFV replication in a dose- and time-dependent manner. PAMs were infected with ASFV (MOI = 1) for 2 h and then treated with compounds at various concentrations. At 48 h (A-C) or at indicated hours post-infection (D, E), samples were collected and detected by HAD50 assay, qRT-PCR assay, or western blotting assay. The virus titer was determined after treatment with brequinar for 48 h (A) or at the indicated hours post-infection (D). The expression level of ASFV-B646L mRNA was analyzed by qRT-PCR at 48 h (B) or at the indicated hours post-infection (E). The expression level of p30 protein was detected by western blotting assay after treatment with brequinar for 48 h (C). Statistical significance is denoted by *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the DMSO-treated control