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Fig. 3 | Virology Journal

Fig. 3

From: Resurgence of SARS-CoV-2 Delta after Omicron variant superinfection in an immunocompromised pediatric patient

Fig. 3

Single amplicon sequencing confirms Delta variant infection with a transient Omicron superinfection. (A) Workflow for Spike amplification, topoisomerase (TOPO) based cloning, and Sanger sequencing of specimens. Created with https://BioRender.com. (B) Quasispecies haplotypes of SARS-CoV-2 Spike region in four selected nasopharyngeal specimens. A maximum likelihood phylogenetic tree of the cloned SARS-CoV-2 Spike fragment sequences was generated from specimens collected on days 4 (light blue; n = 109), 13 (blue; n = 56), 18 (magenta; n = 52), and 31 (cyan; n = 58) since symptom onset. Arrow indicates Delta S gene clone sequence found in the otherwise Omicron-abundant day 18

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Virology Journal

ISSN: 1743-422X