Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Table 8 The effects of Phaseolus vulgaris Endornaviruses 1 and 2 on the accumulation of bean common mosaic necrosis virus (BCMNV) RNA accumulation

Endornavirus Content	Plant Line	Mean relative BCMNV RNA accumulation ± SEM*
Endornavirus-free	GLP24	2.70 ± 0.85^a
	KATX56	0.67 ± 0.14^b
	Wairimu	1.55 ± 0.53^ab
PvEV1	RED40	3.15 ± 1.32^a
PvEV1 & PvEV2	RWR2245	0.022 ± 0.0029^c

^*Reverse transcription-quantitative polymerase chain reaction assays with appropriate primers (Additional file 1: Table S2) were used to measure endornavirus RNA accumulation in twelve cultivars of Phaseolus vulgaris (Additional file 1: Table S1), relative to accumulation of two housekeeping gene transcripts, PvActin 11 and PvUnknown 1 [29] in plants of the indicated common bean lines infected with BCMNV. There were statistically significant differences (p < 0.05) in relative BCMNV RNA steady-state accumulation across the cultivars as determined by Kruskal–Wallis (χ² = 29.275, df = 4, p = 0.000006873, n = 50). Pairwise comparisons were performed using a Wilcoxon rank sum test with Benjamini–Hochberg p-value correction, with statistically significant differences indicated by different lower-case letters. RNA samples were isolated from 10 individual plants of each line to assay for each endornavirus (i.e., n = 10 plants per line per endornavirus). Wairimu = Wairimu Dwarf

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