Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Table 7 The effects of Phaseolus vulgaris Endornaviruses 1 and 2 on the accumulation of bean common mosaic virus RNA accumulation

Endornavirus Content	Plant Line	Mean relative BCMV RNA accumulation ± SEM*
Endornavirus-free	GLP24	0.41 ± 0.071^a
	KATX56	0.77 ± 0.11^b
	Wairimu	6.03 ± 1.44^c
PvEV1	RED40	4.42 ± 1.55^bc
PvEV1 & PvEV2	RWR2245	7.29 ± 2.22^c

^*Reverse transcription-quantitative polymerase chain reaction assays with appropriate primers (Table S2) were used to measure endornavirus RNA accumulation in twelve cultivars of Phaseolus vulgaris (Table S1), relative to accumulation of two housekeeping gene transcripts, PvActin 11 and PvUnknown 1 [29] in plants of indicated lines infected with BCMV. There were statistically significant differences (p < 0.05) in relative BCMV RNA steady-state accumulation across the cultivars as determined by Kruskal–Wallis (χ² = 28.334, df = 4, p = 0.00001067, n = 50). Pairwise comparisons were performed using a Wilcoxon rank sum test with Benjamini–Hochberg p-value correction, with significant differences indicated by different lower-case letters. RNA samples were isolated from 10 individual plants of each line to assay for each endornavirus (i.e., n = 10 plants per line per endornavirus). Wairimu = Wairimu Dwarf

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