Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Table 3 The effects of bean common mosaic virus infection on the accumulation of endornavirus RNAs

Plant Line	Mean relative endornavirus RNA accumulation ± SEM*
	PvEV1		PvEV2
	Mock	BCMV	Mock	BCMV
RED40	1.20 ± 0.23	1.98 ± 0.61	n/a	n/a
RWR2245	1.22 ± 0.26^a	5.52 ± 1.56^b	1.15 ± 0.20^a	3.08 ± 0.81^b

^*Reverse transcription-quantitative polymerase chain reaction assays with appropriate primers (Additional file 1:Table S2) were used to measure endornavirus RNA accumulation in two cultivars of Phaseolus vulgaris (Additional file 1:Table S1), relative to accumulation of two housekeeping gene transcripts, PvActin 11 and PvUnknown 1 [29]. Plants were either infected with bean common mosaic virus (BCMV) or mock inoculated. Statistically significant differences (p < 0.05) in PvEV1 and 2 RNA accumulation between mock-inoculated and infected with BCMV, as determined by Wilcoxon Rank sum test, are indicated by different lower-case letters. RNA samples were isolated from 10 individual plants of each line to assay for each endornavirus (i.e., n = 10 plants per line per endornavirus) with the following exception: RWR2245 mock-inoculated (n = 9)

ISSN: 1743-422X