Fig. 1
From: Safety and immunogenicity of inactivated Rift Valley Fever Smithburn viral vaccine in sheep
Inactivation profile of Rift valley fever virus (RVFV) with binary ethylenimine (BEI). Average titres of the 2-h interval RVFV (Smithburn) inactivated with 4 different concentrations of BEI (0.5 mM, 1 mM, 1.5 mM and 2 mM) were determined. Titres were measured using the tissue culture infectious dose (TCID50) assay. RVFV culture was included and used as a negative control. Complete inactivation of RVFV was first observed at BEI concentration 2 mM after 6 h of treatment, followed by 1.5 mM and 1 mM BEI after 8 h of treatment. Complete inactivation of RVFV by 0.5% BEI was observed after 18 h of treatment. Error bars represent standard error of the mean (SEM)