Fig. 3From: Antibody production and characterization of the nucleoprotein of sever fever with thrombocytopenia syndrome virus (SFTSV) for effective diagnosis of SFTSVIndirect ELISA of produced SFTSV monoclonal antibodies. Anti-SFTSV-N antibodies isolated from immunized mice were used as primary antibodies in indirect ELISA. Purified full-length SFTSV N protein was used as the antigen. A Indirect ELISA using nine SFTSV monoclonal antibodies. Five-fold serial dilutions of 5 µg/mL stock solutions of each antibody were used in the assay. Anti-His6 antibody (▲) and 2B8 antibody (▼) were used as the positive and negative controls, respectively. Anti-His6 antibody applied single concentration (0.1 µg/ml) was indicated together with 5 µg/ml (3.7 as log value) in the figure. B Indirect ELISA of SFTSV antibody clone #1(3H6). Two-fold dilutions of a 200 µg/mL stock solution of 3H6 were used in the assayBack to article page