Fig. 1
From: Isolation and pathogenicity of porcine circovirus type 2 in mice from Guangxi province, China
Identification of PCV2-GX-6 strain. (A) PCR detection of PCV2 in the swine lymph. PCR amplification identified the causative agent using CSFV, PRRSV, PRV, PCV3, and PCV4 specific primers, ddH2O was used as a negative control (Lane 2). (B) Immunofluorescence assay (IFA) of PK-15 cells infected with PCV2-GX-6 strain at 72 h post-infection. The viral protein was detected in PK-15 cells inoculated with PCV2 Cap mAb, and mock-infected cells (PK-15) were used as the negative control. (C) Identification of the different generation isolates by PCR using the specific primer, ddH2O was used as a negative control (Lane 2). (D) Detection of PK-15 cells infected with PCV2-GX-6 strain by Western blot using Cap monoclonal antibody of PCV2. (E) Identification of virus by Transmission electron microscopic shows features of diameter 17nmparticle by negative stain (Bar = 50 nm).