Table 1 Oligonucleotides used in this study
Name | Sequence (5′ to 3′) | Application |
|---|---|---|
F10 S:D614G F | TCTTTATCAGGGTGTTAACTGCAC | Mutagenesis PCR |
F10 S:D614G R | ACAGCAACCTGGTTAGAAGTATTTG | Mutagenesis PCR |
F9 S:E484Q F | CCTTGTAATGGTGTTCAAGGTT | Mutagenesis PCR |
F9 S:E484A F | CCTTGTAATGGTGTTGCAGGTTTTAATTG | Mutagenesis PCR |
F9 S:E484K F | CCTTGTAATGGTGTTAAGGGTTTTAATTG | Mutagenesis PCR |
F9 S:E484Q/A/K R | TGTGCTACCGGCCTG | Mutagenesis PCR |
F9 S:Q498R/N501Y F | GTTTCCGACCCACTTATGGTGTTG | Mutagenesis PCR |
TAR F10b F | CCAACCATACAGAGTAGTAGTAC | TAR fragment |
TAR F10 R | TCATGTTCAGAAATAGGACTTGTTG | TAR fragment [50] |
TAR F9 F | GGAGTCACATTAATTGGAGAAGC | TAR fragment [50] |
TAR F9 R | GCATCAGTAGTGTCAGCAATGTC | TAR fragment [50] |
sgRNA N F | CGATCTCTTGTAGATCTGTTCTC | sgRNA N quantification |
sgRNA N Prb | FAM/ CAG TAA CCA GAA TGG AGA ACG CAG /BHQ | sgRNA N quantification |
sgRNA N R | CAGTATTATTGGGTAAACCTTGG | sgRNA N quantification |
38F | GAAGTCAGACAAATCGCTCCAG | RT-PCR amplicon competition assay |
38R | ACTAGCGCATATACCTGCACC | RT-PCR amplicon competition assay |