Fig. 3

5 × ATF6 reporter activation is dependent on viral biosynthesis in MEFs. A A31 MEFs were transfected with p5xATF6-GL3 and pRL-TK. Cells were exposed to UV-inactivated virus or incubated with arabinose C (AraC) (50 µg/ml) for 30 min prior to virus infection. Incubation was carried in the presence of the inhibitor. Cell lysates were collected and assayed for Firefly and Renilla luciferase activities. Tunicamycin-treated (1 µM) cells lysates were used as positive control. The data are mean of duplicate samples from two independent experiments. *, ** and *** indicate statistically significant differences in relation to untreated cells (p < 0.05; p < 0.01; p < 0.001 respectively) as determined by unpaired two-tailed Student’s T-test