Fig. 1

5 × ATF6 reporter activation kinetics over the course of VACV infection in MEFs. A A31 MEFs were transfected with p5xATF6-GL3 and pRL-TK. and treated with 3 µM of tunicamycin for 0, 6 and 24 h. Cell lysates were collected and assayed for Firefly and Renilla luciferase activities. B Transfected A31 cells were infected with WR or MVA (MOI 10) and harvested at different times points post infection. Asterisks indicate statistically significant differences (p < 0.001) between groups as determined by two-way ANOVA followed by Bonferroni’s test. The data was plotted as means ± SEM of duplicate samples from three independent experiments