Fig. 3

Transcriptional activity of HPV 33 LCR deletion constructs representing different intratype lineages. A: Schematic representation of the different LCR fragments of variants cloned in front of the luciferase gene in the reporter vector pGL2-Basic. B: Relative luciferase activities of HeLa cells transiently transfected with HPV 33 LCR deletion constructs. Luciferase activities are shown relative to the activity of cells transfected with 33LCRHU01 full-length HPV 33 LCR vector construct. Data are from four independent experiments, with standard errors shown as error bars. Statistical analysis was performed using ratio t test. Stars indicate the significance of difference in transcriptional activity compared to 33LCRHU01 or 33LCRHU04 transfected cells within each group of bars as indicated (*p < 0.05, **p < = 0.005, ***p < 0.001)