Fig. 2
Pinacidil reduces IAPV-mediated mortality and IAPV replication in bees. A Data presented as Kaplan–Meier survival curves with data points representing mean survivorship with error bars representing SEM (n = 5, 30 individuals per replicate). Data points for each time point with different letters represent statistical significance as determined by Kaplan–Meier log-rank test with significance designated at P < 0.05. Bees were injected with 26 GEU of IAPV for IAPV only and pinacidil + IAPV groups. B Log10 fold change in IAPV mRNA expression over time following IAPV only or IAPV + pinacidil exposure from whole bees. Bars represent mean (n = 10, 5 individuals per replicate) IAPV genome equivalents. IAPV transcript expression from homogenized bees and error bars represent SEM. C Change in IAPV mRNA expression in dorsal vessels excised from bees 48 h post-infection. Bars represent mean transcript expression and error bars represent SEM (n = 25, 3 individuals per replicate). For B–C, Bars not labeled by the same letters represents statistical significance at P < 0.05 as determined by one-way ANOVA and Tukey’s post