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Table 1 Names, nucleotide sequences and genomic characteristics of RT-RPA-primers and -probe designed to target NSm gene and used in this study for tomato chlorotic spot virus (TCSV) detection

From: Development of a reverse transcription recombinase polymerase amplification combined with lateral flow assay for equipment-free on-site field detection of tomato chlorotic spot virus

Primer/probe name

Sequence (5′–3′)

Length (nt)

GC (%) content

Genome positiona

TCSV_RPA_NSm_F

ATTAATTGACCCTAACATGCCTTCTGACAAGC

32

40.6

414–445

TCSV_RPA_NSm_R

b5Biosg/GCAACACTTATCTTTATCGG CTCTTGGAGAATC

33

42.4

666–634

TCSV_RPA_NSm_Probe

c6-FAM/ATCTAAACTGGTCTATCCCA AAAGCGAACAA/didsp /ACACCTG AAAACTGCTG/e3SpC3

49

42.9

500–530/532–548

  1. aGenome position based on the nucleotide sequence of TCSV (GenBank Accession No. ON783718)
  2. b5Biosg, Biotin
  3. c6-FAM, 6-carboxyfluorescein (Fluorescein)
  4. didsp, internal abasic nucleotide analogue (THF)
  5. e3SpC3, Spacer (polymerase extension blocking group)
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Virology Journal

ISSN: 1743-422X