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Fig. 5 | Virology Journal

Fig. 5

From: Development of a reverse transcription recombinase polymerase amplification combined with lateral flow assay for equipment-free on-site field detection of tomato chlorotic spot virus

Fig. 5

Lateral flow assay (LFA) detections of body heat-mediated RT-RPA assay A Effects of incubation periods on the RT-RPA-LFA detection at 36 °C: lateral flow strip 1–4 with tomato chlorotic spot virus (TCSV) template for 5, 10, 15, and 20 min, respectively; 5-healthy tomato for 15 min. RT-RPA cross-reactivity test using crude extract of symptomatic tomato plants visualized by using B universal lateral flow strips and C LFA detection chambers: lateral flow strip 1 and 2, TCSV; 3, tomato spotted wilt virus (TSWV); 4, tomato brown rugose fruit virus (ToBRFV); 5 tomato necrotic streak virus (TomNSV); 6, healthy tomato-control (HT). C: control line; T: test line

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Virology Journal

ISSN: 1743-422X