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Fig. 4 | Virology Journal

Fig. 4

From: Development of a reverse transcription recombinase polymerase amplification combined with lateral flow assay for equipment-free on-site field detection of tomato chlorotic spot virus

Fig. 4

Specificity and sensitivity of lateral flow assay (LFA) combined with RT-RPA, and its comparison with RT-LAMP assay. A Specificity of RT-RPA-LFA assay used for testing tomato chlorotic spot virus (TCSV) and other tomato infecting viruses. Lateral flow test strip 1, TCSV; 2, tomato spotted wilt virus (TSWV); 3, tomato yellow leaf curl virus (TYLCV); 4, tomato necrotic streak virus (TomNSV); 5, cucumber mosaic virus (CMV); 6, tomato mosaic virus (ToMV); 7, tomato brown rugose fruit virus (ToBRFV); 8, pepino mosaic virus (PepMV); 9, southern tomato virus (STV); 10, healthy tomato (HT); 11, no-template (water) control (WC). B Sensitivity of RT-RPA-LFA in detecting TCSV. Lateral flow test strip 1, total RNA of TCSV (0,6 ug/μl); lanes 2–10, tenfold serial dilutions of total RNA; lane 11, no-template (water) control (WC). C Sensitivity of RT-RPA-LFA in detecting TCSV. Lateral flow test strip 1, crude RNA of TCSV; lanes 2–10, tenfold serial dilutions of crude RNA; lane 11, no-template (water) control (WC). C, control line; T, test line. D Sensitivity of RT-LAMP assay in detecting TCSV using colorimetric endpoint detection with pH indicator (phenol red). Tube one crude RNA of TCSV; tubes 2–10, tenfold serial dilutions of crude RNA; tube 11, no-template (water) control (WC)

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Virology Journal

ISSN: 1743-422X