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Fig. 3 | Virology Journal

Fig. 3

From: Development of a reverse transcription recombinase polymerase amplification combined with lateral flow assay for equipment-free on-site field detection of tomato chlorotic spot virus

Fig. 3

Comparison of specificity and sensitivity of RT-RPA and RT-PCR assays for detection of tomato chlorotic spot virus (TCSV) by agarose gel electrophoresis. A Sensitivity of RT-RPA assay for detection of TCSV. Lane 1, total RNA of TCSV (0.6 ug/μl); lanes 2–10, tenfold serial dilutions of total RNA; lane 11, no-template (water) control; M, 1 kb DNA ladder. B Sensitivity of RT-PCR assay for detecting TCSV using the same RPA primers used in RPA assay in A. Lane 1, total RNA of TCSV (0,6 μg/μl); lanes 2–10, tenfold serial dilutions of total RNA; lane 11, positive control (PC); lane 12, no-template (water) control (WC); M, 1 kb DNA ladder. C Detection specificity of TCSV-specific RT-RPA assay, and D virus specific RT-PCR assays. Lane 1, TCSV; lane 2, tomato spotted wilt virus (TSWV); lane 3, tomato brown rugose fruit virus (ToBRFV); lane 4, pepino mosaic virus (PepMV); lane 5, tomato yellow leaf curl virus (TYLCV); lane 6, tomato necrotic streak virus (TomNSV); lane 7, tomato mosaic virus (ToMV); lane 8, cucumber mosaic virus (CMV); lane 9, southern tomato virus (STV); M, 1 kb DNA ladder

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Virology Journal

ISSN: 1743-422X