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Fig. 2 | Virology Journal

Fig. 2

From: Development of a reverse transcription recombinase polymerase amplification combined with lateral flow assay for equipment-free on-site field detection of tomato chlorotic spot virus

Fig. 2

Optimization of RT-RPA reaction conditions for the detection of tomato chlorotic spot virus (TCSV) that visualized by agarose gel electrophoresis. A Effects of incubation temperatures on the RT-RPA reaction: lanes 1–9 with TCSV RNA template at 30, 33, 35, 36, 37, 38, 39, 40, and 45 °C for 30 min, respectively; lane 10, no-template (water) control at 36 °C; M, 1 kb DNA ladder. B Effects of incubation period on the RT-RPA reaction at 36 °C: lanes 1–9 with TCSV RNA template for 5, 10, 15, 20, 25, 30, 35, 40, and 45 min, respectively; lane 10, no-template (water) control (WC) for 45 min; lane M, 1 kb DNA ladder

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Virology Journal

ISSN: 1743-422X