Fig. 5

Patch-clamp electrophysiology tests for inhibition of SARS-CoV-2 E protein by ivermectin and derivatives. A Current-voltage relation of recombinantly expressed mock controls and 2-E protein. Applied voltages were between −60 to +50 mV. Control currents were taken from mock (pRK vector) transfected HEK293 cells. Panels from left to right show ivermectin (Iv), ivermectin aglycone (IA), milbemycin (Mil) and nemadectin (Nem). Concentrations are indicated in the graphs. B Concentration-response curves: currents at -60 mV were corrected for leak current (mock-transfected cells); the corrected currents observed for 2-E in presence of inhibitor were divided by the corrected currents in absence of inhibitor. Symbols are indicated in the graph. C Summary of the IC₅₀ values of ivermectin derivatives against recombinant 2-E