Fig. 3

Replication kinetics of CCHFV in host cell lines. SW-13 CO2-, SW-13 CO2+ and Vero E6 (VE6) cells were infected with CCHFV, and the culture supernatants were analyzed for viral loads and titers, based on seven independent experiments. There were two replicates per experiment, the mean of which was used as the value representing each experiment (experiment value). Line graphs depict the mean of experiment values from all independent experiments. Bar graph indicates the mean ± SEM of experiment values from all independent experiments. A Viral loads (RNA concentrations) at indicated time points. cp, copies. DPI, days post-infection. Asterisks indicate statistically significant difference found between SW-13 CO2- and SW-13 CO2 + at the corresponding time point: *p < 0.05 and **p < 0.01. B Viral titers (infectious virus concentrations). TCID50, Median Tissue Culture Infectious Dose. All viral titers were determined on SW-13 CO2 + cells using the TCID50 method. Left panel shows viral titers at indicated time points. Right panel compares the peak viral titers. Both the SW-13 CO2− and SW-13 CO2+ cultures demonstrated significantly higher peak viral titers than the VE6 culture: *p < 0.05. The CO2+ culture shows trend of a higher peak viral titer than CO2− (p = 0.14)