Skip to main content

Table 7 Comparison of the duplex and quadruplex SARS-CoV-2 PCR assays using 24 weakly positive patient samples

From: Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic

Performance parameter

Duplex assay

Quadruplex assay

Number of detected samples

20/24

22/24

 E-Gene and orf1ab detected

18/24

17/24

 Only E-Gene detected

0/24

4/24

 Only orf1ab detected

2/24

1/24

E-Gene mean Ct value

34.03

33.55

E-Gene range of Ct values

28.94–37.97

27.67–38.70

orf1ab mean Ct value

35.37

34.02

orf1ab range of Ct values

29.47–43.41

28.63–41.58

  1. 24 weakly positive patient samples were tested by using either the E-Gene/KoMa and orf1ab/c-myc duplex PCR assays or the E-Gene/KoMa/orf1ab/c-myc quadruplex assay
Back to article page

Virology Journal

ISSN: 1743-422X