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Table 5 PCR positivity comparison between five automated RNA extraction methods by using serial diluted cell culture supernatant

From: Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic

Sample

Dilution factora

Q-Con

Q-HT

Q-Sym

KFF

MP96

1

–

4/4

4/4

4/4

4/4

4/4

2

1:2

4/4

4/4

4/4

4/4

4/4

3

1:4

4/4

4/4

4/4

4/4

2/2*

4

1:10

4/4

4/4

4/4

4/4

4/4

5

1:20

4/4

4/4

4/4

4/4

4/4

6

1:40

4/4

4/4

3/4

4/4

4/4

7

1:100

3/4

3/4

1/4

2/4

3/4

8

1:200

0/4

0/4

1/4

1/4

1/2*

9

1:400

0/4

1/4

1/4

0/4

1/4

10

1:1000

0/4

1/4

0/4

0/4

0/4

11

1:2000

0/4

0/4

1/4

0/4

0/4

  1. PCR positivity comparison was performed by using serial diluted cell culture supernatant of SARS-CoV-2. The number of positive rRT-PCR replicates is shown for each sample. The SARS-CoV-2 rRT-PCR assay targets two regions of the SARS-CoV-2 genome and was performed in duplicate, yielding a total of four PCR replicates per RNA extract
  2. Q-Con: QIAcube Connect, Q-HT: QIAcube HT, Q-Sym: QIAsymphony, KFF: King Fisher Flex, MP96: MagNa Pure 96
  3. aThe dilution factor relative to the most highly concentrated sample 1 is given in order to allow a relative comparison of performance around the limit of detection. In the pre-tests, sample 1 had a Ct value of 30 in both the E-Gene and orf1ab rRT-PCR assay
  4. *RNA analysis not performed in duplicate due to insufficient volume of RNA extract
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Virology Journal

ISSN: 1743-422X