Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic

Table 4 Primers and probes for all FAM quadruplex PCR reactions: E-Gene/KoMa/orf1ab/c-myc

Name	Sequence	O^a	Position^b	Tm^c
E_Sarbeco_F1*	ACAggTACgTTAATAgTTAATAgCgT	S	26,141	53.9
E_Sarbeco_R2*	ATATTgCAgCAgTACgCACACA	A	26,253	57.9
E_Sarbeco_P1*	FAM-ACACTAgCCATCCTTACTgCgCTTCg-BHQ1	S	26,204	65.0
E TMGB add	FAM-CTTgCTTTCgTggTATT-MGB	S	26,171	67.0
KoMa F	ggTgATgCCgCATTATTACTAgg	S	n/a^d	57.8
KoMa R	ggTATTAgCAgTCgCAggCTT	A	n/a^d	57.8
KoMa TM	TexRed-TTCTTgCTTgAggATCTgTCgTggATCg-BBQ	S	n/a^d	67.7
orf1ab S	CTCTggAACACTTTTACAAgACTTC	S	19,608	54.5
orf1ab A	ACCATCAACTTTTgTgTAAACAgTg	A	19,754	56.3
orf1ab TMGB	FAM-ACAgggTgAAgTACCA-MGB	S	19,688	66.0
orf1ab add	FAM-TAATgTTgTAAATAAgggACAC-MGB	S	19,641	66.0
c-myc F	TAgTggAAAACCAgCAgCCT	S	380	57.0
c-myc R	TCgTCgCAgTAgAAATACgg	A	488	56.0
c-myc TM	Cy5-TATgACCTCgACTACgACTCggTgC-BBQ	S	442	63.5

^aOrientation
^bPosition in GenBank entry (E_Sarbeco: Acc# NC_004718; orf 1ab: Acc# MN997409.1; c-myc NM_002467.6, GeneID: 4609)
^cThermodynamic melting temperature
^dArtificial sequence
*Corman et al. [8]

ISSN: 1743-422X