Table 3 Master mix for quadruplex PCR reaction: E-Gene/KoMa/orf1ab/c-myc

Water

–

2 × RT-PCR buffer

–

nCoV E_Sarbeco F1 (100 µM)

400

nCoV E_Sarbeco R2 (100 µM)

400

E_Sarbeco P1 (100 µM)

200

KoMa F (100 µM)

300

KoMa R (100 µM)

300

KoMa TM (100 µM)

100

orf1ab S (100 µM)

300

orf1ab A (100 µM)

300

orf1ab TMGB (100 µM)

100

orf1ab add (100 µM)

100

c-myc F (100 µM)

120

c-myc R (100 µM)

120

c-myc TM (100 µM)

100

25 × RT-PCR Enzyme Mix

–

Mix Vol

RNA Vol

Total Vol