Table 1 Details of RNA extraction and purification methods
Instru-ment (manufacturer) | Kit | Instrument protocol | Lysis and inactivation procedure | Sample volume (µl) | Elution volume (µl) | Conc. factor (sample vol. to elution vol.) |
|---|---|---|---|---|---|---|
QIAcube Connect (Qiagen) | QIAamp Viral RNA Mini | QIAamp Viral RNA_Body fluid_Manual lysis custom-12a | 560 µl of buffer AVL + 560 µl of ethanol (off-board) | 140 | 60 | 2.3 |
QIAcube HT (Qiagen) | QIAamp 96 Viral RNA | QIAamp 96 Viral RNA OBL Protocol (high vacuum)b | 560 µl of buffer AVL + 560 µl of ethanol (off-board) | 140 | 80 | 1.75 |
QIAsym-phony SP (Qiagen) | QIAsymphony DSP Virus/Pathogen | ComplexOBL_2019-nCoVa | 560 µl of buffer AVL + 560 µl of ethanol (off-board) | 140 | 60 | 2.3 |
MagNA Pure 96 (Roche) | MagNA Pure 96 DNA and Viral NA Small Volume Kit | Viral NA Plasma ext lys SV 4.0 | 250 µl of external lysis buffer (off-board) | 200 | 50 | 4 |
KingFisher Flex (Thermo Fisher Scientific) | MagMAX Viral/Pathogen Nucleic Acid Isolation | MVP_2Wash_200_Flex | 0.25Â mg of Proteinase K (on-board) | 200 | 50 | 4 |