Fig. 4
From: Cross-reactive immune responses to monkeypox virus induced by MVA vaccination in mice
Humoral immune responses induced by various protective antigens of MPXV. BALB/c mice (n = 5) were immunized twice with different PAs or mixed PAs with or without AddaVax adjuvant, and 107 TCID50/mL MVA as a positive control. On day 35 postimmunization, sera were collected to determine humoral immune responses induced by various MPXV PAs. (A) The titers of IgG antibody against MVA were evaluated by ELISA assay. MVA strain was used as ELISA antigen. Comparing the levels of IgG antibody among the adjuvanted antigen group and the non-adjuvanted antigen group, the asterisk * indicates a significant difference (P < 0.05) detected by T-test analysis. (B) MVA-neutralizing antibody titers were determined by PRNT. Comparing the vaccination effect among MPXV PAs and PBS groups, the asterisk * indicates a significant difference (P < 0.05) detected by one-way ANOVA.