Fig. 5

Oxamate does not affect LCMV infectious virion production. MRC-5 cells were infected with LCMV at an MOI of 3. After adsorption, the inoculum was removed and replaced with complete medium or complete medium supplemented with oxamate (25 or 50 mM). A Twenty-four hours after medium exchange, cell viability was measured by LIVE/DEAD™ Fixable Dead Cell Stain Kit. Data from four independent experiments are shown as a scatter of individual values. Mean and SD are shown as column and error bars, respectively. B LCMV titers in the extracellular media were determined by a focus-forming assay. Data from three independent experiments are shown as a scatter of individual values. Mean and SD are shown as column and error bars, respectively. Statistical differences between groups were analyzed using Welch’s ANOVA with Tamhane’s T2 post hoc test (A) or one-way ANOVA and Tukey’s post hoc test (B). Different letters above the columns indicate significant differences (P ≤  < 0.05), while identical letters indicate no significant difference. C The relative (%) inhibitory effect of oxamate on LCMV infection was calculated from LCMV titers shown in B (for more details, see Materials and methods). Data are shown as a mean and SD. D Simplified schematic representation of glycolysis and tricarboxylic acid cycle with highlighted target of oxamate. FFU/ml, focus-forming units per milliliter