Fig. 3

Neither glucose consumption nor extracellular lactate production is affected during LCMV infection. A MRC-5 cells were mock- or LCMV-infected at an MOI of 3. Cell lysates were harvested at indicated time after infection, and glucose uptake was measured using the Glucose Uptake-Glo™ Assay. B MRC-5 cells were infected as described above, cell culture medium was collected at the indicated time after infection, and the relative lactate amount was measured using the Lactate-Glo™ Assay. Data from three to four independent experiments are shown as a scatter of individual values. Mean and SD are shown as column and error bars, respectively. Statistical differences between groups were analyzed using one-way ANOVA with Sidak’s post hoc test. ns, not significant; hpi, hours post-infection; RLU, relative light unit