Fig. 6

Validation of DEPs by Western blot analysis and qRT-PCR. A The expression levels of FLNA, TXN, and CYCS in Mock or HEV-infected HepG2 cells supplemented with FBS, NPS, or PS were analyzed through Western blot analysis at 4 and 24 hpi. GAPDH served as the loading control. B The relative expression levels of FLNA, TXN, and CYCS were analyzed and normalized to the expression level of GAPDH. C Knockdown of FLNA by shRNA validated by Western blot. D The relative gene expression of RIG-I in cells infected with HEV or transfected with shRNA targeting FLNA. E The copy number of HEV in HEV-infected cells transfected with or without shRNA targeting FLNA. Three independent experiments were preformed. Student’s t-test (two-tailed) was used to compare differences between two groups. *p < 0.05, **p < 0.01, ***p < 0.001