Fig. 5
From: Identification of small molecules capable of enhancing viral membrane fusion
Analysis of hit compounds in the presence of TMPRSS2. A–F The ability of the selected compounds to increase syncytia formation in the presence of TMPRSS2 was tested with the BiMuC assay. Hek293T cells were transfected with TMPRSS2 alongside the viral and cellular machinery required for syncytia formation. Next, cells were mixed and treated with the appropriate compound for 48 h. The fluorescence percentages over the mock (DMSO) treated samples are represented. The average and standard deviation of at least three independent experiments are shown. Dots represent individual experiments. Based on a one-tailed homoscedastic t-test, statistical differences between compound and mock-treated samples were calculated. When differences are significant, the p-value is shown