Fig. 3

Analysis of hit compounds. A–F Selected compounds were repurchased, and their ability to increase syncytia formation was tested with the BiMuC assay. Briefly, Hek293T cells were transfected with Jun plus SARS-CoV 2 S proteins and independently with Fos and human ACE2. After 24 h, samples were combined and treated with the appropriate compound at the indicated concentration or mock treated for 48 h. Next, the fluorescence, indicative of the percentage of fusion was calculated. The fluorescence percentages over the mock (DMSO) treated samples are represented. The average and standard deviation of at least three independent experiments are shown. Dots represent individual experiments. Based on a one-tailed homoscedastic t-test, statistical differences between compound and mock-treated samples were calculated. The p-value is shown when significant differences are found