Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China

Table 3 Presumed VP4 neutralizing antigen epitope differences between N4006 and vaccines or P[8] strains circulating globally

Strains	8 − 1											8 − 2		8 − 3									8 − 4			5 − 1								5 − 2	5 − 3	5 − 4	5–5
Strains	100	146	148	150	188	190	192	193	194	195	196	180	183	113	114	115	116	125	131	132	133	135	87	88	89	384	386	388	393	394	398	440	441	434	459	429	306
N4006	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
Z6398/2019/CHN/G9P[8]-E2 ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
Fuzhou18-152/2018/CHN/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
JS2016/2016/CHN/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
Km15100/2015/CHN/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
SC9/2014/CHN/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
Tokyo18-43/2018/JPN/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
VU12-13-101/2013/USA/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
JES11/2010/ITA/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	G	I	T	A	N	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
CAU09-376/2017/KOR/G9P[8] ^a	D	S	Q	D	S	T	N	L	N	D	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
Moscow-40/2020/RUS/G3P[8] ^b	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
Dhaka16/BGW/2003/G1P[8] ^b	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
CAU17L-103/2009/KOR/G8P[8] ^b	D	S	Q	D	S	T	N	L	N	G	I	T	A	D	P	V	D	N	R	N	D	N	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
RV1/G1P[8] ^c	D	S	Q	E	S	T	N	L	N	N	I	T	A	N	P	V	D	S	S	N	D	N	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
RV5/P[8] ^c	D	S	Q	E	S	T	N	L	N	D	I	T	A	N	P	V	D	N	R	N	D	D	N	T	N	Y	F	L	W	P	G	R	T	P	E	L	R
Rotavin-M1/P[8] ^c	D	S	Q	E	S	T	N	L	N	D	I	T	A	S	P	V	D	N	R	N	D	D	N	T	N	Y	F	I	W	P	G	R	T	P	E	L	R
LLR/P[15] ^d	D	T	N	T	Y	T	N	Y	D	S	V	T	A	P	E	T	T	T	A	N	P	Q	T	S	E	Y	F	L	W	P	G	R	T	P	D	L	R
Rotavac-116E/P[11] ^d	T	S	A	A	G	Y	N	V	P	N	A	D	G	A	Q	T	S	T	D	N	S	S	S	N	D	Y	F	L	W	P	G	G	T	P	Q	C	R
RV5/P[5] ^d	G	A	D	D	Y	V	N	Y	A	S	V	T	A	T	S	E	T	S	S	N	A	D	T	G	P	Y	F	L	W	P	G	R	T	P	E	L	R

VP4 consists of two subunits, VP8* and VP5*, which have neutralizing epitope regions. The presumed neutralizing antigen epitopes in VP8* (8 − 1, 8 − 2, 8 − 3, 8 − 4) and VP5* (5 − 1, 5 − 2, 5 − 3, 5 − 4, 5–5) of N4006 were compared with those of vaccine strains and RVA P[8] epidemic strains. ^a Prevalent strains of G9P[8] genotype in VP4 phylogenetic tree lineage 3; ^b prevalent strains of P[8] of other G genotypes in lineage 3; ^cP[8] genotype vaccines; ^d vaccines of other P genotypes; bold numbers, antigen-escape-related neutralizing epitopes; bold letters, changes in antigen epitopes of reference strains compared with N4006. For prevalent strains of P[8] belonging to other G genotypes in lineage 3, only a few strains were used as references

ISSN: 1743-422X