Fig. 1
From: Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China
Characteristics of N4006 cultured in MA104 cells. (A) Morphology of RVA by TEM. (B) PAGE map of dsRNA genomic fragments of RVA strains SA11, RotaTeq W179-9 and N4006 of different generations. (C) Green fluorescence (20× magnification) was detected in MA104 cells at 24 h after infection with N4006. a, green fluorescence diagram of MA104 cells infected with N4006; b, MA104 cells infected with N4006 using light microscopy. (D) Growth curve of N4006. MA104 monolayer cells were infected with N4006 and SA11 (MOI 0.01). Cell lysates after repeated freeze-thawing were collected every 12 h, and titers were determined by DAB colorimetry. Results are the means of three determinations; error bars indicate standard deviation. (E) The CPE induced by RVA N4006 in MA104 cells. a, MA104 cells infected with N4006 with CPE; b, MA104 cells uninfected with rotavirus