Table 1 Primers used for PRV gE/gI gene and TK gene knockouts
Gene | Primer name | Primer sequence (5’→3’) | length (bp) |
|---|---|---|---|
gI-L | gI-LF | GGGGTACC(KpnI)GACGCCGCACCGCCCCTTC | 796 |
| Â | gI-LR | CCGCTCGAG(XhoI)GGCACCAGGGCCGTCAGCAC | |
gE-R | gE-RF | CCCAAGCTT(HindIII)CGCGCCCGTGGAACCCGTA | 1162 |
| Â | gE-RR | GGACTAGT(SpeI)GCCCCGTCCATCAGCGTGACCA | |
TK-L | TK-LF | GGGGTACC(KpnI)CCGCCTTATCATCCCCGCTCCCC | 694 |
| Â | TK-LR | CCGCTCGAG(XhoI)CCATCGGCTCGGGCACGTACAG | |
TK-R | TK-RF | CCCAAGCTT(HindIII)GGTGTGACCCTCGCCCCTC | 854 |
| Â | TK-RR | GGACTAGT(SpeI)TCGCGCATCGTCTGGTGCAT | |
EGFP | EGFP-F | CCGCTCGAG(XhoI)ACTCTGTTCGACACGGACAC | 1709 |
| Â | EGFP-R | CCCAAGCTT(HindIII)ATCTCCGACGTGAAGGCGCA | Â |