Table 3 Calculation of PCR efficiency using ORF1ab Ct values for EQA samples
RT-PCR Kits | Three dilutions | Four dilutions | ||||
|---|---|---|---|---|---|---|
1/slope | E (%) | r2 | 1/slope | E (%) | r2 | |
BioGerm | − 0.238 | 72.98 | 0.9913 | − 0.3723 | 135.67 | 0.8586 |
DaAn | − 0.28 | 90.55 | 0.9902 | − 0.2917 | 95.75 | 0.9956 |
BGI | − 0.2519 | 78.61 | 0.9614 | − 0.2561 | 80.34 | 0.9863 |
Geneodx | − 0.2478 | 76.93 | 0.9995 | − 0.2947 | 97.11 | 0.9824 |
Kinghawk | − 0.2083 | 61.55 | 0.9834 | − 0.2641 | 83.70 | 0.9575 |
Hybribio | − 0.35 | 123.87 | 0.9682 | − 0.4022 | 152.46 | 0.9753 |
Maccura | − 0.3584 | 128.24 | 0.9618 | − 0.3694 | 134.10 | 0.9858 |
EasyDiagnosis | − 0.3254 | 111.54 | 0.9839 | − 0.3307 | 114.14 | 0.9943 |
Nagene | − 0.2213 | 66.46 | 0.9985 | − 0.3253 | 111.49 | 0.9009 |
Sansure | − 0.329 | 113.30 | 0.9572 | − 0.3277 | 112.67 | 0.9855 |
Bioperfectus | − 0.2951 | 97.29 | 0.9999 | − 0.3609 | 129.56 | 0.976 |
Zybio | − 0.2676 | 85.18 | 0.9857 | − 0.3531 | 125.48 | 0.9444 |