Fig. 1

The Ct values derived from EQA samples were detected by different RT-PCR kits. a The Ct values determined by different RT-PCR kits combined with different extraction kits and different PCR instruments, b the Ct values determined by different RT-PCR kits combined with the Tianlong nucleic extraction kit and different PCR instruments, c the Ct values determined by different RT-PCR kits combined with the DaAn nucleic extraction kit and different PCR instruments, d the Ct values determined by different RT-PCR kits combined with Tianlong nucleic extraction kit and ABI7500 PCR instrument