Fig. 6

Images of virus-infected cells and RT-qPCR results. A Viral infected cells. GiCF cells were infected with the supernatant of strain YC-01 stored in our laboratory, and sampled at different timepoints (0 h, 12 h, 24 h, 36 h, 48 h, 54 h, 60 h, 66 h, 72 h, 78 h, 84 h, 90 h, 96 h, 108 h and 120 h). Over time, CPE of cells gradually became obvious until all cells died. B–E RT-qPCR results. RNA was extracted by Trizol reagent and reverse-transcribed to cDNA to be used as template. The above genes were quantified using a real-time instrument. Error bars represent standard deviation of the mean for experiments performed in triplicate (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). F Virus titre analysis. Titre values after viral infection of cells were determined at different timepoints