Fig. 2

PCR amplification, prokaryotic expression, and validation of the ORF55 gene. A DNA extracted from the virus supernatant of infected cells was used as the amplification template, and the ORF55 gene was amplified by PCR with specific primers. M, DNA Marker 2000; 1, MBP gene (partial); 2, ORF55 gene; 3, MBP-ORF55 gene; 4, negative control. B Induced expression analysis of the ORF55 protein. The bacterial solution before and after IPTG induction was collected by centrifugation and analysed by SDS-PAGE. M, Protein markers; 1, uninduced; 2, induced. C SDS-PAGE analysis. The recombinant ORF55 protein was purified and the collected fractions were subjected to SDS-PAGE. The target protein was purified to a high concentration, and molecular weights of 45 kDa and 70 kDa were observed for bands corresponding to the MBP tag and ORF55 recombinant protein, respectively. M, Protein markers; 1, MBP protein; 2, ORF55 recombinant protein. D Western blotting analysis of purified ORF55 recombinant protein. A single band was observed. M, Protein markers; 1, MBP protein; 2, ORF55 recombinant protein