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Fig. 8 | Virology Journal

Fig. 8

From: ARIH1 inhibits influenza A virus replication and facilitates RIG-I dependent immune signaling by interacting with SQSTM1/p62

Fig. 8

ARIH1 up-regulated the expression of RIG-I by interacting with SQSTM1. A ARIH1 was overexpressed or silenced in HEK293T cells, and the expression of endogenous RIG-I was detected by Western blot assay after stimulation with SeV. B HEK293T cells were co-transfected with pFlag-ARIH1 and HA-tag SQSTM1 plasmid (pHA-SQSTM1), and immunoprecipitation assay was performed using Flag tag or HA tag antibody to analyze their interaction. C HEK293T cells were co-transfected with pHA-SQSTM1 and pFlag-ARIH1 or the corresponding empty vectors and then stimulated with SeV after 24 h. Western blot assay was performed using antibodies specific for RIG-I, p-IRF3, Flag-tag, HA-tag, and GAPDH

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Virology Journal

ISSN: 1743-422X