Fig. 7
From: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions
Nsp2 may affect composition of the cap-binding complexes. (A) Left, the representative images of western-blot analyses of proteins expressed in control and Nsp2-cells under normal and hypoxic conditions. Arrow indicates position of Strep-Nsp2. Right, ratio of densitometric signal of P-4EBP to total 4EBP in cells grown under normal or hypoxic conditions normalized to that in control cells grown under normal conditions, set as one (± SD, n = 3). (B) Left, the representative images of western blot analysis of proteins bound to m7GTP-Sepharose under normal and hypoxic conditions in control and Nsp2-cells. Arrow indicates position of Strep-Nsp2. Right, ratios of densitometric signals of eIF4G (upper graph) and eIF4E2 (lower graph) to eIF4E bound to m7GTP-Sepharose in experiments presented in lanes 1–4 (± SD, n = 3). Graph eIF4E/eIF4G: p = 0.014 for C–H versus C–N; p = 0.033 for Nsp2-H versus C–H. Graph eIF4E2/eIF4E: p = 0.03 for C–H versus C–N; p = 0.025 and 0.023 for Nsp2–H versus C–N and versus C–H, respectively