Fig. 1

Amplification results for the FCV and FHV-1 target fragments. The amplified PCR products were electrophoresed in a 1.5% (W/V) agarose gel for 30 min at 120 V. (A) Lanes 1 and 2 are the target fragments required for the construction of pMD-FCV. (B) Lanes 1 and 2 are the target fragments required for the construction of pMD-FHV-1. Screening of optimal FCV (C, E) and FHV-1 (D, F) primer pairs