Fig. 8
MoMo30-plant binds to purified gp120. A Gp120 was bound to a Biacore chip surface, and MoMo30-plant was allowed to flow across the chip at concentrations from 1 to 100 nM. Changes in surface plasmon resonance monitored binding. B Gp120 pre-treated with PNGase F (an N-linked glycosylase) dramatically reduces binding. The three lines represent triplicate measurements. C Mannose can block the activity of MoMo30-plant. HIV-1NL4-3, 2 nM of MoMo30-plant, and different concentrations of D-mannose were added simultaneously and incubated for 5 min at 37 °C before testing by MAGI cell assay for inhibition of infection. Inhibition as a percentage of the untreated control (Infection in the presence of 2 nM Momo30-plant) is plotted