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Fig. 6 | Virology Journal

Fig. 6

From: Anti-human immunodeficiency virus-1 activity of MoMo30 protein isolated from the traditional African medicinal plant Momordica balsamina

Fig. 6

In vitro translation of the MoMo30-plant gene produces a 30 kD protein with antiviral activity. A An in vitro synthesized gene was inserted into a pGenLenti vector and used as a template for coupled transcription/translation. The reaction was run on a 20% SDS-PAGE gel, and a western blot was probed with an N-terminal ab to MoMo30-plant. B One hundred µL of the reaction mix was tested in a MAGI assay. C The MoMo30-plant pGenLenti plasmid was used to transfect HEK 293 cells. Supernatant and cell lysates were run on a 20% SDS-PAGE gel and probed with the N-terminal ab. A sample of purified MoMo30-plant is used as a marker. D One hundred µL of the cell-free conditioned medium as tested by MAGI assay

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Virology Journal

ISSN: 1743-422X